Journal: Cellular and molecular life sciences : CMLS

Article Title: Multiple circulating forms of neprilysin detected with novel epitope-directed monoclonal antibodies.

doi: 10.1007/s00018-023-05083-1

Figure Lengend Snippet: Fig. 4 Antibody validation by western blot analysis. Reactiv- ity of mAbs (indicated above each panel) to a recombinant human sNEP (Aviscera Biosci- ence) and Trx-AgNEP(1–4) was determined using western blot performed with automated Jess system (ProteinSimple). The ability of AgNEP-3 (b) and AgNEP-4 (c) mAbs to detect endogenous human NEP was determined by probing them against NEP-positive LNCaP cell lysate (L) and NEP-neg- ative PC-3 cell lysate (P). PE pAb was used as the positive control. Ladder: 12–230 kDa biotinylated molecular weight ladder (ProteinSimple). The primary target band of each protein sample type is indicated with a black arrow. Image exposure setting: High Dynamic Range 4.0 except for the Trx-AgNEP(1–4) panel where Exposure 5: 16 secs was used

Article Snippet: The reactivity of the mAbs to various protein sample types was determined by simple western analysis using the Jess fully automated system (ProteinSimple; Bio-Techne, USA) following the manufacturer’s instructions.

Techniques: Biomarker Discovery, Western Blot, Recombinant, Positive Control, Molecular Weight